Fluorescence Mini Cube with 4 ports – Excitation, Fluorescence and Opsin Activation

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The diagram shows measurement involving an excitation, an optogenetic activation/silencing, a fluorescence detection and a sample ports. Such a cube can be used for GCaMP fluorescence measurements combined with the activation of red opsins in the 580-650 nm band. On the drawing E is for excitation, F for fluorescence, O for opsin activation/silencing and S is for fixed sample port. The numbers in the brackets of the ordering code are for the corresponding wavelength bands.