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Fluorescence Mini Cube with 6 ports – Two-fluorophore Fluorescence and Lock-in Autofluorescence Detection

Fluorescence Mini Cube with 6 ports - Two-fluorophore Fluorescence and Lock-in Autofluorescence Detection

 FMC6_AE(405)_E1(465-480)_F1(500-540)_E2(555-570)_F2(580-680)_S

This cube version can separate the fluorescence from two fluorophores, and has an additional channel for a lock-in autofluorescence detection channel.

 

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Another possibility is to use three excitation wavelengths and to detect the fluorescence from two indicators and the autofluorescence from the sample on one of the two detectors. The separation of the fluorescence signals from autofluorescence signal is possible if the light sources are modulated.



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