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Basic Snap-in Fluorescence Microscope Bodies

The Basic Snap-in Fluorescence Microscope Body is offered in two models: S or L. Both models have the dichroic beam-splitter, M3 optical connector, CMOS sensor etc. Each CMOS has a serial number stored within its cable that points to a specific set of mask correction filters recognizable to our software package. Model L has a 0.5 NA objective lens within its body while Model S has a plan-parallel plate instead and relies on the objective lens within the model S imaging cannula to create an image on the CMOS. When used for deep brain imaging, the fluorescence microscope body is used with an implantable imaging cannula that transfers the image from its bottom to its top.

Notes:

• The ultralight fiberglass jacket is lighter and more flexible, the lightweight metal jacket is more robust but heavier.
• The standard electrical cable length is 1000 mm. Other values on request (up to 3000 mm).
• The optical fiber length is adjusted to fit the desired electrical cable length.
• Every microscope body comes with a protective cap.



Microscope brain zones





SFMB spectra

The next figure shows the fluorescence intensity traces of 16 cells expressing GCaMP6 in the D2 receptor-expressing medium spiny neurons in the indirect pathway of striatum. The movie was acquired in the striatum (about 4 mm deep) in a freely-moving mouse. Courtesy of Dr. Akiyo Natsubori (experimenter), and Dr. Kenji Tanaka (PI) from the Department of Neuropsychiatry, School of Medicine, Keio University.


Calicum traces measured with the Doric Image Analyzer